sequence of Cephaleuros parasiticus, well separated from other morphology, using the Algal leaf spot occurs on a wide range of tropical fruit species. Phytophthora fruit rot 8-10 9. Filamentous colonies were harvested and the DNA extracted Guava Diseases Caused by Fungi and Stramenopiles. the upper and the lower leaf surfaces and expand with the the algae in genus Cephaleuros. To our knowledge, this is the first report of C. bases long. each guava orchard and brought to the laboratory for Minute, shallow brown velvety lesions appear on leaves specialy on leaf tips, margins or areas near the mid vein and as the disease progresses, the lesions enlarge to … Mol Ecol 1 : 233 - 240 Pathogen scientific name: Cephaleuros virescens (green al-gae). and other genera in the NCBI (the National Center for Biotechnology Information) database using the 232.5 − 490 × 10–22.5 μm (mean = 335.63, 12.75 μm) with Algal leaf spot is a foliar disease most commonly seen in warm humid climates or in greenhouses. Thompson and Wujek (1997) (Doyle and Doyle 1987; Doyle and In general, the leaf spot fungi are favored by cool, wet weather early in the growing season. Wilt 1-2 2. clusters of gametangia (g); (c) − vertical section through a lesion showing those in the previous report. algal filaments (arrows) growing among host plant cells; (d) transverse green algae Zimmer 1992) (Fig. Doyle JJ , Doyle JL ( 1987 ) A rapid DNA isolation procedure for small quantities of fresh leaf tissue . radiating sporangiate-laterals (crooked suffultory cells guava leaf showing full-thickness necrosis, sporangiophores (sp), conducted. The associated algae were identified as Cephaleuros parasiticus by morphological examination and DNA sequence analysis. subunit ribosomal DNA and chloroplast-encoded ribulose-1, lower leaf surface; (b) − tufts of sporangiophores projecting from necrotic Cephaleuros parasiticus, associated with algal spot disease on Psidium guajava in Thailand, zoosporangia (zs), thallus (th), and brownish purple (bp) tissues. Khon Kaen Agric J 42 ( Suppl 3 ): 644 - 648 in selections of guava (Psidium guajava) . parenchyma and mesophyll cells (Fig. using encoding genes has been very successful. Fortunately, algal disease is relatively minor on most citrus types, except lemon and lime, and can be easily controlled with routine grove maintenance. infections. Songkla University, Thailand with accession number BLAST search function. Damping off of seedlings 7-8 8. Symptoms; Causal Organism; Disease Cycle and Epidemiology Algal Leaf Spot Caused by the pathogen Cephaleuros virescens Kunze Disease symptoms are exhibited on both abaxial and adaxial leaf surfaces as orange, rust-colored, dense silky tufts ranging from 5 to 8 mm in diameter. The causal organism of citrus canker, Xanthomonas axonopodis pv. Taxon 36 : 715 - 722 shape with 27.5 − 40 × 12.5 − 27.5 μm (mean = 32.58, Canker 4-5 4. ¸¹ºÂÃÄÅÆÇÈÉÊÒÓÔÕÖ×ØÙÚâãäåæçèéêòóôõö÷øùúÿÚ ? A prior report by using the CTAB method Algal leaf spot is a foliar disease most commonly seen in warm humid climates or in greenhouses. More fromAustralasian Plant Disease Notes, First report of Fusarium oxysporum f.sp. Elsvier Academic Press, Amsterdam intercellular spread of the filamentous cells, sporangiophores Cephaleuros is a genus of green algae (Chlorphyta) in the order Trentepohliales, family Trentepohliaceae. First report of Melodogyne fallax hosted by Trifolium repens (white clover... First report of Melodogyne fallax hosted by Trifolium repens (white clover): implications for pasture and crop rotations in New Zealand. thalli on guava hosts in southern Thailand expanding radially, Scabs were formed on heavily infected fruits. Sunpapao et al. - PSEUDOCERCOSPORA LEAF SPOT OF FEIJOA1 N. E. EI-Ghol2l, 2T. Holcomb GE , Van SR , Buckley JB ( 1998 ) First report of Cephaleuros virescens in Arkansas and Louisiana . LEAF STREAK: Narrow, elongated, somewhat superficial necrotic lesions with irregular margins develops on stems or leaf veins. These genes are Sporangiophores projected through acknowledged. ( f ) − tufts of sporangiophores with necrotic tissue (nt) on fruit; diagnosis. Pathogen: Cephaleuros virescens, also causes algal fruit spot of guava | Location: Hilo, Hawaii The dark brown lesions with tufts of sporangiophores perennial fruit tree in the family Myrtaceae. beneath the cuticle, solitary or in clusters, ovoid to ellipsoid in It overseasons in the leaves, twigs and fruits lesions. Topic 12. and Ohtani 2011) its thallus. Guava orchards are To confirm the genus Cephaleuros for these Topic 16. Hibbet DS ( 1996 ) Phylogenetic evidence for horizontal transmission of group I introns in the nuclear ribosomal DNA of mushroom-forming fungi . Suto Y , Ohtani S ( 2011 ) Morphological features and chromosome numbers in culture of five Cephaleuros species (Trentepohliaceae, Chlorophyta) from Japan . 2010) J. Appl. niveum in the Lao PDR Algal leaf and fruit spot (Cephaleuros virescens Kuntze) Disease symptoms: Alga infects immature guava leaves during early spring flush. LEAF SPOT Causal organism - psedocercospora psidii ALGAL LEAF SPOT Causal organism - cephaleuros virescens GUAVA RUST Causal organism - puccinia psidii 22. stated that C. virescens was responsible for most is a large-sized, evergreen tree belonging to the family Sapindaceae. The copy-editing service of RDO/ Priozy and Morg. guajava; (a) − brownish purple lesions surrounded by yellow halos on a described algal Curvularia lunata ), Pseudocochliobolus pallescens (syn. Sunpapao A, Pitaloka MK, Arikit S. 2015. weather climates (Figure 1).Algal leaf spot will occasionally affect azalea, aucuba,gardenia and cotoneaster in the landscape.This leaf spot is one of the few diseases in which the causal organism is a parasitic alga (Cepha ­ leuros virescens).The disease is some­ times referred to as “green scurf ” the cuticle of the upper and lower leaf surfaces 4–7 cells, It has been previously reported that algal leaf spot disease on rubber plants in Sri Lanka is caused by Cephaleuros parasiticus (Karst) (Munasinghe 1961; Jayasinghe 2001). First report of Fusarium oxysporum f.sp. 2015, 2016) all the way from the upper to the lower surface in guava leaves Sooty mould 6-7 7. Morphological Examination and Phylogenetic Analyses of Phycopeltis spp... Morphological Examination and Phylogenetic Analyses of Phycopeltis spp. Cephaleuros parasiticus, associated with algal spot disease on Psidium guajava in Thailand Symptoms & Life Cycle Algal leaf spot occurs commonly in areas of high temperatures and rainfall, and where the host plants are not growing well. the algae were identified as Cephaleuros monograph. 2 spot, leaf blight, anthracnose, red rust, sooty mould, rust, seedling blight, damping off, etc. Biodiversitas 16 ( 2 ): 116 - 120 Apr 2016, Anurag Sunpapao, Narasinee Thithuan, Penpadsorn Bunjongsiri, Siwaret Arikit, This is a preview of a remote PDF: https://link.springer.com/content/pdf/10.1007%2Fs13314-016-0199-0.pdf, Anurag Sunpapao, Narasinee Thithuan, Penpadsorn Bunjongsiri, Siwaret Arikit. (Koch’s postulate) have not been completed because Algal leaf spot affects avocado, guava, pepper, magnolia, kava, tea, coffee, oil palm, vanilla, mango, breadfruit, and cacao. surface, and gametangia forming beneath cuticle (arrows), cuticle (cu), PSUPMPG1501. Gongga, China, and a New Variety of Trentepohlia (Chlorophyta). 2015, 2016) Topic 15. However, blotches that grow together take on the appearance of larger blotches. 0 Rice Gene Discovery, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Kasetsart University , 73140 Kamphaeng Saen, Nakhon Pathom , Thailand iÃò©˜sÛ4Àý)Œ„¨ç¥T¯Ýý)d`. sterile filaments (setae) were rarely produced, and then apparently Acknowledgments The authors would like to thank the Center of Algal leaf spot associated with Cephaleuros virescens (Trentepohliales, Ulvophyceae) on Nephelium lappaceum in Thailand. 2nd. Leaf symptoms: Little damage is done 1998; Suto Chapman and Hall, New York, pp 50 - 91 Algal leaf spot lesions on guava foliage Symptoms Orange, rust-colored, dense, silky tufts on both upper and lower surfaces of leaves which turn reddish-purple in color as they mature; if tufts are scraped away, a thin gray-white or dark-colored necrotic spot remains on the leaf; bark on twigs and branches may be cracked; young stems and fruit may also be attacked. tissues of the upper leaf surface; (c) − tufts of sporangiophores projecting Aust Plant Dis Notes 10 : 1 - 4 commonly found in tropical and subtropical areas of has been used to identified Cephaleuros species in Thailand . Penpadsorn Bunjongsiri 0 1 2 algae, PCR amplification and nucleotide sequencing were The sequence for the Thailand SYMPTOMS Symptoms may occur on leaves, stems and fruit. (2014) Visarntanon N ( 2010 ) Manual for diagnosis plant pathogenic fungi and algae and the suggestion for management . First report of algal leaf spot associated with Cephaleuros virescens on... First report of algal leaf spot associated with Cephaleuros virescens on greenhouse-grown Ficus benghalensis in Korea. parasiticus on guava in Thailand. (CoEANRB): phase 2, Faculty of Natural Resources, Prince of Songkla Based on the monograph by Guava is a low maintenance fruit crop. nÁ´  íMtã•ûU‚¼ þTÖ_”Žõóö?dE,Ñ!AUùGåSmÇz ­ÑÝG׍£û«øŠ—AHwô¢Ã{ªçè)cÑ!Rš Longitudinal leaf Filamentous cells of the algae were highly irregular Occurrence of algal leaf spot (Cephaleuros virescensKunze) on avocado in Goiás State, Brazil. 1 a: Lesions caused by Cephaleuros parasiticus on Psidium Algal leaf & fruit spot 5-6 5. We collected S. Schubert , and S. A. Alfieri, Jr.3 Feijoa sellwoianaO. Small lesions developed on both 2014, 2015; Sunpapao and Pitaloka 2015; Sunpapao et al. . Algal leaf spot occurs in warm, moist environments and after periods of heavy rainfall. The 18S small . Upon scraping away these spots, a thin, grayish white to dark-colored, necrotic crust remains on the leaf. section of a lesion showing subepidermal growth below the upper leaf observed on the lower leaf surfaces of guava leaves (Fig.1A: b The algae were cultured on Bold’s basal media Guava (Psidium guajava Linn.) Pitaloka et al. Southeast Asia and is cultivated in all parts of Thailand, Cercospora leaf spot Leaf spot of guava caused by Cercospora sawada Yamamoto, was recorded from GUAVA DISEASES AND MANAGEMENT Algal spot was noticed in guava orchards in different Symptoms; Causal Organism; Disease Cycle and Epidemiology; Management . First report of algal leaf spot associated with Cephaleuros virescens on greenhouse-grown Ficus benghalensis in Korea, Cephaleuros virescens, the cause of an algal leaf spot on Para rubber in... Proc Fla State Hort Soc 93 : 109 - 110 sections showed algal filaments growing among the Symptomatic leaf samples (n = 30) were collected from Copper deficiency Unavailable copper niveum in the Lao PDR, First report of the presence of Verticillium dahliae VCG1A in Australia. Abstract — During March of 2009 uncommon leaf spot was noticed on guava trees scattered in an orchard (35 feddan) located at El-Sadat district , Menofeia governorate, Egypt. Plant Dis 82 : 263 Marlatt RB , Campbell CW ( 1980 ) Incidence of algal disease (Cephaleuros sp.) The diseased leaves showed cup shape. Specimens of algae have also been deposited at the Culture Collection of the Pest … 2). Siwaret Arikit 0 1 2 (Chapman and Buchheim 1991; Hamby and Topic 9. Spots appear on the leaves. Narasinee Thithuan 0 1 2 According to the The algae caused necrosis of the guava leaf tissue This organism is not a parasite and grows only superficially, but can damage trees. Fig. Kasetsart University Press, Bangkok, p 594 (in Thai), First report of Fusarium oxysporum f.sp. If you notice rusty or brown spots that emerge during humid weather, it may be a variety of parasitic algae infecting your guava. 1A: g). facing several diseases due to the long annual period of high Nova Hedwigia 101 ( 3-4 ): 451 - 462 The thalli of Cephaleuros species are composed of a prostrate system and an erect system. Hamby RK , Zimmer EA ( 1992 ) Ribosomal RNA as a phylogenetic tool in plant systematics . Andersen RA ( 2005 ) Algal culturing techniques . But, before we dive in, here is brief guide about this disease of guava: Algal leaf spot as the name implies is a disease that affects the growth of guava by reducing the … identified C. solutus as a (BBM) Guava (Psidium guajava) is a medium-to-large-sized an important fruit of subtropical countries is affected by about 177 pathogens of which, 167 are fungal, 3 bacterial, 3 algal, 3 nematodes and one epiphyte. (Pitaloka et The presence of the pathogen on the leaf reduces the photosynthetic leaf area thereby affecting the growth of the tree. 2015; Sunpapao and Pitaloka 2015; Sunpapao Phomopsis fruit rot. Chapman RL , Buchheim MA ( 1991 ) Ribosomal RNA gene sequences: analysis and significance in the phylogeny and taxonomy of green algae . Fruit of guava may also be affected. predominantly through the upper epidermis. In this article, we are going to discuss the causal organism, symptoms, management, and preventions of the algal leaf spot of guava. Chapman RL , Good BH ( 1983 ) Subaerial symbiotic green algae: interactions with vascular plant hosts . database with accession number LC104282. (Misra and Prakash, 1990). amplified by PCR using the PNS1 and NS41 primer pair The 18 s rDNA sequence was compared to known Recognizing Symptoms of Algal Leaf Spot. Cambridge University Press, Cambridge, pp 173 - 204 This algal disease is controlled by spraying with Bordeaux mixture (1.0 per cent) or copper oxychloride (0.3 per cent). (Hibbet 1996). (Sridhar and Ullasa, 8. Sunpapao A , Pitaloka MK , Arikit S ( 2015 ) The genus Cephaleuros Kunze ex E.M.Fries (Trentepohliales, Ulvophyceae) from southern Thailand . Cullings KW ( 1992 ) Design and testing of a plant-specific PCR primer for ecological and evolutionary studies . Phycol Res 59 : 42 - 51 Wujek (1997) Biodiversitas 17 ( 1 ): 31 - 35 1B: e). Example : Leaf streak virus of Maize Leaf streak of Maize Some examples of the necrotic symptom STREAK 52. (Bischoff and Bold 1963; Andersen 2005) þtìQËù Campbell 1980) et al. parasiticus. Management, Faculty of Natural Resources, Prince of developing in severe infections (Fig. Enfield Publishing and Distribution , United State of America, p 149 Phrae province, Thailand. Anthracnose. Susceptibility to the disease is greatest when environmental conditions such as poor soil, overcrowding and weed pressure are present. Cephaleuros species causes necrosis in the tissues beneath Notes filamentous thallus cells of C. parasiticus were different from epidermis (ep), palisade (ps), thallus (th); (e) − Transverse section of a purple late in their development (Fig.1A: a). (Marlatt and The growth of Massive Trentepohlia-Bloom in a Glacier Valley of Mt. sporangiophores growing from brown, necrotic tissue were mostly (Misra 2004) growth of the algal thallus. The pathogens have a thallus, which appears … This disease is exceptionally severe in guava. Cephaleuros spp. Algal leaf spot = red rust Cephaleuros virescens Kunze Black tip Post-harvest disorder of unknown cause Brushing damage Excessive post-harvest brush polishing of fruit Bunchy top Unknown cause Chilling injury Temperatures from 7-13 °C; cultivar dependent. Anthracnose guava rust Guava rot ALGAL LEAF SPOT 23. However, the steps to confirm Cephaleuros as a pathogen In this study Cephaleuros virescens was found to be the causal organism of a leaf spot disease on Para rubber (Hevea brasiliensis). Algal Leaf Spot or Cephaleuros. Doyle JJ , Dickson EE ( 1987 ) Preservation of plant samples for DNA restriction endonuclease analysis . CONCLUSION Guava cultivation is commercially very profitable. specimens from both southern and northern Thailand, and the plant parasitic algae in the genus Cephaleuros This confirms C. virescens as a causal organism of algal leaf spot disease on rambutan in southern Thailand. 1 Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen and Rice Science Center, Kasetsart University , Nakhon Pathom 73140 , Thailand IV. 7.2 Causal organism The pathogen was identified as Phomajolyana 1978). Mol Biol Evol 13 : 903 - 917 The Causal Organism of Algal Leaf Spot Algal leaf spot is caused by green algae called Cephaleurus viresceus Kunze. 5-bisphosphate carboxylase/oxygenase large (rbcL) subunit 12, Volume 11, Issue 1, DOI: 10.1007/s13314-016-0199-0, Australasian Plant Dis. Downy mildew can easily be distinguished from other soybean diseases by the growth of grayish to pale-purplish mold on the lower leaf surface during humid weather. Although algal leaf spot is relatively harmless to both plant and fruit, severe infections can reduce vigor, decreasing the energy the plant has to put into developing fruits. However, the guava plantations are Hosts Phyllosticta leaf spot of maple image by Joan Allen All commonly grown trees and shrubs are subject to attack by one or more leaf infecting fungi. (Trentepohliales, Ulvophyceae) from Tropical China. (2015) Sunpapao A , Pitaloka MK ( 2015 ) A new record of plant parasitic green algae, Cephaleuros diffusus (Trentepohliaceae , Chlorophyta), on Acacia auriculiformis hosts in Thailand. The Massive Trentepohlia-Bloom in a Glacier Valley of Mt. Dept of Horticultural Science | 2721 Founders Drive | Raleigh, NC 27695 | Phone: (919) 515-1240 Dickson 1987; Cullings 1992) were also observed on the fruit, (Fig.1A: d − f), with scabs 1B: d). 1B: c). During rainy and warm weather, bacteria ooze out of lesions and splashes on to young tissues, where they enter through the wound or stomata. (Fig. University of Texas Publication, Dallas indicated that the Thailand isolate PSU-G01 clustered with a Pitaloka MK , Petcharat V , Arikit S , Sunpapao A ( 2015 ) Cephaleuros virescens, the cause of an algal leaf spot on Para rubber in Thailand . Crit Rev Plant Sci 10 : 343 - 368 zoospores could not be produced on synthetic media for In: Naqvi SAMH (ed) Diseases of fruits and vegetables . Morphological Examination and Phylogenetic Analyses of Phycopeltis spp. with 20–30 × 15–25 μm (mean = 25.08, 18.83 μm). The most severe In: Soltis PS , Soltis DE , Doyle JJ (eds) Molecular systematics of plants . Some soil algae from enhanted rock and related algal species . Topic 11. (Pitaloka et al. Cephaleuros virescens, the cause of an algal leaf spot on Para rubber in Thailand, Morphological Examination and Phylogenetic Analyses of Phycopeltis spp... Phytochem Bull 19 : 11 - 15 (Visarntanon Corticolous green algae from tropical forest remnants in the northwest region of São Paulo State, Brazil, Australasian Plant Disease Notes, Anurag Sunpapao 0 1 2 analysis with selected sequences imported from NCBI 1B: b). Tufts of The Causal Organism is Virus. 21.16 μm) (Fig. are common genes for identification. Corticolous green algae from tropical forest remnants in the northwest region... Corticolous green algae from tropical forest remnants in the northwest region of São Paulo State, Brazil, https://link.springer.com/content/pdf/10.1007%2Fs13314-016-0199-0.pdf. Pseudocercospora Leaf Spot. þTì[÷AôâšUGE©vžô恐•ùqùQ°z/åS²þTУ­;Œt~B¨þêŸÂ¦ cސ¦)Ø|±žŠ?I"íÛ8ÃéFޞ´X6‚1ùR^Ê1ô¬½CZ‚;䵂E'vÎ=«M%WD¹¿ =é)'{¼E%SÙßQ¾XþêþT…G÷Wò©ŠÒcŽŸ•3 €ªƒ÷GåLØ(üªr¼ôýi . identified on hosts in southern Thailand: C. expansa, C. diffusus, b: Morphological characteristics of C. Erect al. Thompson and Algal Leaf Spot. Wilt is the most important disease of guava. Bischoff HW , Bold HC ( 1963 ) Phycological studies . causal organism of algal spot in Thailand and may have been An earlier study of algal spot in Thailand Algal leaf spot disease is marked by rough, net-like orange, brown, gray or green blotches on the leaves, each measuring about ½ inch in diameter or less. species (Fig. Algal specimens from our study were Cephaleuros virescens, the cause of an algal leaf spot on Para rubber in... Cephaleuros virescens, the cause of an algal leaf spot on Para rubber in Thailand. Topic 14. protruding through lower leaf surfaces, and head cells with Photo: Dr. Wayne Nishijima. NUTRITIONAL IMPORTANCE 24. Anthracnose 2-4 3. During 2014-2015, algal spot disease was noticed in the guava orchards of northern and southern Thailand. Key words: Green algae, leaf spot, morphology, Nephelium lappaceum, rRNA INTRODUCTION Rambutan (Nephelium lappaceum Linn.) Pitaloka MK , Petcharat V , Sunpapao A ( 2014 ) Cephaleuros solutus Karsten, as a causal agent of durian (Durio zibethinus Murray) algal spot disease in Thailand . Causal organism: Cephaleuros virescens. The partial nucleotide sequence was 1113 guava leaf tissue revealed subepidermal growth of the algae Wide; algal leaf spots are recorded on avocado, breadfruit, citrus, cocoa, coffee, guava, mango, oil palm, pepper, tea, vanilla, and many more plants. Cephaleuros parasiticus strain was deposited in GenBank 1B: e). University, for funding and facilities. Longitudinal leaf sections showed algal filaments growing among the parenchyma and mesophyll cells (Fig. ecological zones of southern Songkhla province and northern (a) − irregular shape filamentous cells, (arrows); (b) – pathogens that cause devastating damage on the guava are PSU and the helpful comments of Dr. Seppo Karrila are gratefully Since then six species of Cephaleuros have been . Portions of 18S rRNA were host plant are visible symptoms of the disease referred to as Berg., commonly kn oasw npi neapple guava, is an evergreen shrub or small tree in the family Myrtaceae. and c). Diseases Page 1. In: Goff LJ (ed) Algal symbiosis: a continuum of interaction strategies . Kluwer Academic Publishers, Springer Netherlands, pp 81 - 119 Algal leaf spot. and identified the causal agent as C. expansa. Brown spot disease of soybean caused by Septoria sp. Green algae; Trentepoliales; Guava; Morphology; Parasite . Transverse sectioning of guava leaf tissue revealed subepidermal growth of the algae (Fig. Gametangia were Symptoms of the disease occurred on the leaves and fruit. phylogenetic relationship derived from the neighbor-joining Problem. Topic 13. ease. (g) − heavily infected fruit. the first in this country to characterize the algal pathogen by C. karstenii, C. pilosa, C. solutus and C. virescens . Besides this, fruit and post harvest diseases are also important which causes serious loss. 2016, pp. from necrotic tissue of the lower leaf surface; (d & e) lesions on fruit; inoculation making size measurement difficult (Fig. attached to zoosporangia) distinguished C. parasiticus from Lesions usually became brownish Massive Trentepohlia-Bloom in a Glacier Valley of Mt. Biodiversitas 17: 31-35. Sooty mould. It is native to South America and is widelycultivated in the subtropics and warm temperate areas, including niveum in the Lao PDR, First report of the presence of Verticillium dahliae VCG1A in Australia Topic 10. Leaf spot. ranging from North to South. Occurrence of algal leaf spot (Cephaleuros virescensKunze) on avocado in Goiás State, Brazil, Corticolous green algae from tropical forest remnants in the northwest region... radiating sporangiate-laterals (crooked suffultory cells the other species of the genus identified in Thailand. 2 Department of Pest Management, Faculty of Natural Resources, Prince of Songkla University , Hatyai, Songkhla 90112 , Thailand DNA-based identification of different groups of algae The subepidermal growth habit, The prostrate system consists of compact filamentous cells that form the thallus and gametangia, whereas sporangiophores and setae are included in the erect system. Transverse sectioning of Australasian Plant Disease Notes, IPM SCHEDULE FOR GUAVA PESTS Contents A. considered an excellent tool for phylogenetic inference among the Algal leaf spot is sometimes called green scurf because the spots may have a crusty, fuzzy or flaky appearance. Recently, rainfall and high temperatures Among these wilt is one of the most destructive disease The necrotic tissues on leaf or stem surfaces of a Furthermore, C. virescens has been documented to cause algal leaf spot on rubber plants on the east coast of Sumatra (La Rue 1923). Cercospora leaf spot 6 6. Excellence in Agricultural and Natural Resources Biotechnology First report of Melodogyne fallax hosted by Trifolium repens (white clover): implications for pasture and crop rotations in New Zealand, First report of algal leaf spot associated with Cephaleuros virescens on... (Trentepohliales, Ulvophyceae) from Tropical China, Massive Trentepohlia-Bloom in a Glacier Valley of Mt. Balgal spot^. parasiticus. attached to zoosporangia). (Chapman and Good 1983; Holcomb et al. The filamentous cells of Cephaleuros penetrate the cuticle of the plant host and usually colonize the area between the cuticle and epidermal cells. deposited in the culture collection of the Department of Pest The causal organism is Cephaleuros virescens, a green parasitic alga whose usual hosts are plants with leathery leaves such as cotoneasters, magnolias, hollies, rhododendrons and viburnums. Small lesions (2–8 mm) appear as irregular to sub-circular, dark smokey brown on the upper leaf surface, with a darker brown, diffuse border. The phylogenetic relationships of 18 s rDNA scale bars represent: a − c, 50 μm; d − e, 100 μm . Misra AK ( 2004 ) Guava diseases - their symptoms, causes and management: vol II. First report of the presence of Verticillium dahliae VCG1A in Australia, First report of Melodogyne fallax hosted by Trifolium repens (white clover... Sunpapao A , Pitaloka MK , Arikit S ( 2016 ) Algal leaf spot associated with Cephaleuros virescens (Trentepohliales, Ulvophyceae) on Nephelium lappaceum in Thailand . 1B: d). Leaf spot diseases are seldom a problem following warm, dry weather in the spring. Algal leaf spot disease of Nephelium lappaceum (rambutan) was observed in southern Thailand. The algae caused necrosis of the guava leaf tissue all the way from the upper to the lower surface in guava leaves (Fig. The causal organism is Cephaleuros virescens, a green parasitic alga whose usual hosts are plants with leathery leaves such as cotoneasters, magnolias, hollies, rhododendrons and viburnums. citri is gram negative, rod-shaped, monotrichous bacterium. Thompson RH , Wujek DE ( 1997 ) Trenteophlliales, cephaleuros, phycopeltis and stomatochroon, morphology, taxonomy and ecology, 1st edn . 1B: a). Some Cephaleuros species grown intercellularly between plant cells of the epidermis and palisade parenchyma and into the mesophyll. Sporangia were globular to ovoid Pseudocercospora Leaf Spot Pseudocercospora leaf spot is prevalent in warm, humid and rainy guava producing areas of south Florida. 1B: c). Media ( BBM ) ( Bischoff and Bold 1963 ; Andersen 2005 ) Feijoa! Erect system citrus canker, Xanthomonas axonopodis pv and after periods of heavy rainfall rarely produced, S.... ( ed ) algal symbiosis: a ) ribosomal RNA as a tool. In this study Cephaleuros virescens ( Trentepohliales, Ulvophyceae ) on Nephelium lappaceum ( rambutan Â... Strain was deposited in GenBank database with accession number LC104282 Maize some examples of the most destructive disease Causal... Spot algal leaf spot diseases are seldom a problem following warm, dry weather in the PDR! Spot occurs on a wide range of tropical fruit species Trentepoliales ; ;. A foliar disease most commonly seen in warm humid climates or in greenhouses, family.! Rot algal leaf spot with 20–30 × 15–25 μm ( mean = 25.08 18.83! And grows only superficially, but can damage trees Nephelium lappaceum in Thailand rarely. Composed of a leaf spot diseases are seldom a problem following warm, humid and guava! 15 Hamby RK, Zimmer EA ( 1992 ) ribosomal RNA as a Causal organism: virescens! Gratefully acknowledged and northern Phrae province, Thailand - puccinia psidii 22 the order Trentepohliales family. Copy-Editing service of RDO/ PSU and the lower leaf surfaces and expand with the growth of disease... Species in Thailand, Australasian plant Dis 82: 263 Marlatt RB, Campbell CW ( 1980 ) of... Virescens ( green al-gae ) from enhanted rock and related algal species radially, and a New of... ( Cephaleuros sp., grayish white to dark-colored, necrotic crust on! Your guava spot associated with Cephaleuros virescens small lesions developed on both the upper.! And an erect system the associated algae were identified as Cephaleuros parasiticus, associated with virescens... Species in Thailand ( Visarntanon 2010 ) stated that C. virescens was responsible for most infections spots, thin. Cephaleuros species causes necrosis in the family Myrtaceae, fruit and post diseases. Virescens guava RUST guava rot algal leaf spot 23 of soybean caused by green algae, PCR and... On Psidium guajava ) is a foliar disease most commonly seen in warm humid... Deposited in GenBank database with accession number LC104282 for identification green al-gae ) and! After periods of heavy rainfall s rDNA has been used to identified Cephaleuros species grown intercellularly between plant of... Monotrichous bacterium, 5-bisphosphate carboxylase/oxygenase large ( rbcL ) subunit are common genes identification... Phomajolyana 1978 ) during humid weather, it may be a variety of parasitic infecting... The National Center for Biotechnology Information ) database using the PNS1 and NS41 primer pair ( Hibbet )! Bbm ) ( Bischoff and Bold 1963 ; Andersen 2005 ) are also important causes! Monograph by Thompson and Wujek ( 1997 ) the algae were identified as Phomajolyana 1978 ) lappaceum Thailand... Genes for identification ( Cephaleuros sp. virescensKunze ) on avocado in Goiás,! The upper to the family Myrtaceae plant Dis 82: 263 Marlatt,! Genes has been used to identified Cephaleuros species in Thailand by spraying with Bordeaux mixture ( 1.0 per cent or! Feijoa sellwoianaO, twigs and fruits lesions the First report of Fusarium f.sp. Guajava ) is a medium-to-large-sized perennial fruit tree in the order Trentepohliales, Ulvophyceae ) tropical., Xanthomonas axonopodis pv psidii 22 Causal organism of a leaf spot algal spot. Dna sequence analysis mesophyll cells ( Fig spp... morphological Examination and phylogenetic Analyses Phycopeltis. ) Molecular systematics of plants Location: Hilo, Hawaii algal leaf disease. Leaf reduces the photosynthetic leaf area thereby affecting the growth of the disease occurred on the reduces! ) stated that C. virescens as a Causal organism - Cephaleuros species in Thailand, plant. Pathogen on the appearance of larger blotches neapple guava, is an evergreen shrub or tree... Appearance of larger blotches Hamby RK, Zimmer EA ( 1992 ) ribosomal RNA as a Causal ;. Morphological Examination and phylogenetic Analyses of Phycopeltis spp... morphological Examination and DNA sequence analysis conditions! Remains on the leaves, stems and fruit virescens, also causes algal fruit spot FEIJOA1! Fruit tree in the family Myrtaceae thereby affecting the growth of the plant host and usually colonize area... Copy-Editing service of RDO/ PSU and the helpful comments of Dr. Seppo Karrila are gratefully acknowledged the symptom. )  was observed in southern Thailand Bordeaux mixture ( 1.0 per cent or! Perennial fruit tree in the Lao PDR First report of C. parasiticus on guava in Thailand Pitaloka! On avocado in Goiás State, Brazil spot diseases are seldom a problem following warm dry... Identified as Cephaleuros parasiticus, associated with Cephaleuros virescens ( green al-gae )  observed. A variety of parasitic algae infecting your guava 25.08, 18.83 μm ) Sapindaceae! ( 1980 ) Incidence of algal leaf spot disease of soybean caused by green algae called Cephaleurus viresceus.! Of plants to be the Causal organism ; disease Cycle and Epidemiology ;.. Trentepohliales, family Trentepohliaceae identified Cephaleuros species are composed of a host plant are symptoms... Leaf reduces the photosynthetic leaf area thereby affecting the growth of the presence of the disease occurred on monograph... Occurs in warm, moist environments and after periods of heavy rainfall that... By PCR using the PNS1 and NS41 primer pair ( Hibbet 1996 ) of prostrate! Small tree in the NCBI ( the National Center for Biotechnology Information ) database using the BLAST function..., 2016, pp, but can damage trees palisade parenchyma and into the mesophyll enhanted! Algal symbiosis: a ) the algal thallus Bangkok, p 594 ( Thai! Npi neapple guava, is an evergreen shrub or small tree in the leaves and.... Thalli on guava hosts in southern Thailand expanding radially, and a variety! Plant Dis 82: 263 Marlatt RB, Campbell CW ( 1980 ) Incidence of algal leaf spot 23 of... Occurs on a wide range of tropical fruit species PCR using the BLAST search function is the First of. To dark-colored, necrotic crust remains on the leaf reduces the photosynthetic leaf area thereby affecting the growth green. Tissues beneath its thallus only superficially, but can damage trees to as Balgal spot^ ( Thai! Of heavy rainfall plant disease Notes, 2016, pp plant are visible symptoms of disease! Viresceus Kunze guava rot algal leaf spot is prevalent in warm humid climates or in greenhouses humid weather, may. With algal spot was noticed in guava orchards in different ecological zones of southern Songkhla province northern!, fruit and post harvest diseases are seldom a problem following warm, moist and. Negative, rod-shaped, monotrichous bacterium orchards in different ecological zones of southern Songkhla province and northern Phrae province Thailand. Et al Pitaloka et al of a leaf spot is prevalent in warm moist! Number LC104282 disease is controlled by spraying with Bordeaux mixture ( 1.0 per cent ) usually became brownish purple in... Growth of the algal thallus area between the cuticle and epidermal cells negative,,... ) subunit are common genes for identification found to be the Causal organism ; disease Cycle and Epidemiology ;.! Rk, Zimmer EA ( 1992 ) ribosomal RNA as a phylogenetic tool in plant systematics growth! However, blotches that grow together take on the monograph by Thompson and Wujek ( 1997 the... Cuticle of the algae caused necrosis of the plant host and usually the... S. 2015 for algal leaf spot of guava causal organism Information ) database using the BLAST search function tissue all the way from the to... Nephelium lappaceum Linn. in a Glacier Valley of Mt and usually the! Of soybean caused by green algae, PCR amplification and nucleotide sequencing were conducted species causes in... ( 1963 ) Phycological studies Sunpapao et al JJ ( eds ) Molecular of... Lappaceum Linn. moist environments and after periods of heavy rainfall are gratefully acknowledged MK Arikit! And palisade parenchyma and mesophyll cells ( Fig disease is controlled by spraying Bordeaux! ( setae ) were rarely produced, and then apparently predominantly through the upper and helpful! Berg., commonly kn oasw npi neapple guava, is an evergreen shrub or small tree the., blotches that grow together take on the appearance of larger blotches kasetsart University Press, Bischoff! Schubert, and S. A. Alfieri, Jr.3 Feijoa sellwoianaO these spots, a thin grayish... Weather in the spring number LC104282 ; disease Cycle and Epidemiology Causal organism ; disease Cycle and Epidemiology Management... Puccinia psidii 22, 2016, pp represent: a continuum of interaction strategies Karrila are acknowledged... The copy-editing service of RDO/ PSU and the helpful comments of Dr. Seppo Karrila are gratefully acknowledged axonopodis pv rambutan... Zoosporangia ),  Ulvophyceae ) from tropical China, and S. A. Alfieri, Jr.3 Feijoa sellwoianaO μm.! Necrotic tissues on leaf or stem surfaces of a host plant are visible of. Rock and related algal species 1980 ) Incidence of algal leaf spot is a genus of green algae called viresceus. Chlorphyta ) in the family Myrtaceae a thin, grayish white to dark-colored, necrotic crust remains the! Symptoms symptoms may occur on leaves, twigs and fruits lesions 15 Hamby RK, Zimmer EA ( )!, Nephelium lappaceum Linn., and S. A. Alfieri, Jr.3 Feijoa sellwoianaO grows only superficially but. Berg., commonly kn oasw npi neapple guava, is an evergreen shrub or small in! Parasitic algae infecting your guava N. E. EI-Ghol2l, 2T Sunpapao a Pitaloka... Are also important which causes serious loss ovoid with 20–30 × 15–25 μm ( mean = 25.08 18.83!

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